HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >

Purification and properties of phospholipase A2 isozymes from pyloric ceca of the starfish (asterina pectinifera)

Files in This Item:
PA2_Isozymes.pdf448.08 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/8339

Title: Purification and properties of phospholipase A2 isozymes from pyloric ceca of the starfish (asterina pectinifera)
Authors: Kishimura, Hideki Browse this author →KAKEN DB
Hayashi, Kenji Browse this author
Issue Date: Aug-2004
Publisher: Blackwell
Journal Title: Journal of Food Biochemistry
Volume: 28
Issue: 3
Start Page: 181
End Page: 194
Abstract: Phospholipase A2 isozyme II (PLA2 II) which showed different mobility on native PAGE from that of the PLA2 isozyme I (PLA2 I) isolated previously was purified from pyloric ceca of the starfish (Asterina pectinifera). The PLA2 II was mainly released oleic acid from 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. N-terminal amino acid sequence of the PLA2 II was SVYQF. Temperature and pH optimums of the PLA2 II were at around 50C and pH 9.0, respectively, and the enzyme activity was enhanced by sodium deoxycholate and 1 mM or higher concentration of Ca2+. The PLA2 II did not show the fatty acid specificity for hydrolysis of phosphatidylcholine (PC). Specific activity of the PLA2 II was about 10 times higher than that of commercially available porcine pancreatic PLA2. The PLA2 II hydrolyzed PC more effectively than phosphatidylethanolamine. These characteristics of the PLA2 II were the same as those of the PLA2 I.
Description: The definitive version is available at www.blackwell-synergy.com
Relation: http://www.blackwell-synergy.com/loi/jfbc
Type: article (author version)
URI: http://hdl.handle.net/2115/8339
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 岸村 栄毅

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University