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A dual enhancer-silencer element, DES-K16, in mouse spermatocyte-derived GC-2spd(ts) cells

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Title: A dual enhancer-silencer element, DES-K16, in mouse spermatocyte-derived GC-2spd(ts) cells
Authors: Bandara, Thusitha A. M. K. Browse this author
Otsuka, Kai Browse this author
Matsubara, Shin Browse this author
Shiraishi, Akira Browse this author
Satake, Honoo Browse this author
Kimura, Atsushi P. Browse this author →KAKEN DB
Keywords: Dual enhancer-silencer
Multifunctional genome
Spermatocyte
GC-2spd(ts) cell
ChIP-sequencing
Histone modification
Issue Date: 1-Jan-2021
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 534
Start Page: 1007
End Page: 1012
Publisher DOI: 10.1016/j.bbrc.2020.10.049
Abstract: The multifunctionality of genome is suggested at some loci in different species but not well understood. Here we identified a DES-K16 region in an intron of the Kctd16 gene as the chromatin highly marked with epigenetic modifications of both enhancers (H3K4me1 and H3K27ac) and silencers (H3K27me3) in mouse spermatocytes. In vitro reporter gene assay demonstrated that DES-K16 exhibited significant enhancer activity in spermatocyte-derived GC-2spd(ts) and hepatic tumor-derived Hepa1-6 cells, and a deletion of this sequence in GC-2spd(ts) cells resulted in a decrease and increase of Yipf5 and Kctd16 expression, respectively. This was consistent with increased and decreased expression of Yipf5 and Kctd16, respectively, in primary spermatocytes during testis development. While known dual enhancer silencers exert each activity in different tissues, our data suggest that DES-K16 functions as both enhancer and silencer in a single cell type, GC-2spd(ts) cells. This is the first report on a dual enhancer-silencer element which activates and suppresses gene expression in a single cell type. (C) 2020 Elsevier Inc. All rights reserved.
Rights: ©2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/83746
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 敦

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