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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Multiple Fatty Acid Sensing Mechanisms Operate in Enteroendocrine Cells
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| Title: | Multiple Fatty Acid Sensing Mechanisms Operate in Enteroendocrine Cells |
| Authors: | Hira, Tohru Browse this author →KAKEN DB | | Elliott, Austin C. Browse this author | | Thompson, David G. Browse this author | | Case, R. Maynard Browse this author | | McLaughlin, John T. Browse this author |
| Issue Date: | 2004 |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 279 |
| Issue: | 25 |
| Start Page: | 26082 |
| End Page: | 26089 |
| Publisher DOI: | 10.1074/jbc.M400098200 |
| Abstract: | Fatty acids (FA) with at least 12 carbon atoms increase intracellular Ca2+ ([Ca2+]i) to stimulate cholecystokinin release from enteroendocrine cells. Using the murine enteroendocrine cell line STC-1, we investigated whether candidate intracellular pathways transduce the FA signal, or whether FA themselves act within the cell to release Ca2+ directly from the intracellular store. STC-1 cells loaded with fura-2 were briefly (3 min) exposed to saturated FA above and below the threshold length (C8, C10, and C12). C12, but not C8 or C10, induced a dose-dependent increase in [Ca2+]i, in the presence or absence of extracellular Ca2+. Various signaling inhibitors, including d-myo-inositol 1,4,5-triphosphate receptor antagonists, all failed to block FA-induced Ca2+ responses. To identify direct effects of cytosolic FA on the intracellular Ca2+ store, [Ca2+]i was measured in STC-1 cells loaded with the lower affinity Ca2+ dye magfura-2, permeabilized by streptolysin O. In permeabilized cells, again C12 but not C8 or C10, induced release of stored Ca2+. Although C12 released Ca2+ in other permeabilized cell lines, only intact STC-1 cells responded to C12 in the presence of extracellular Ca2+. In addition, 30 min exposure to C12 induced a sustained elevation of [Ca2+]i in the presence of extracellular Ca2+, but only a transient response in the absence of extracellular Ca2+. These results suggest that at least two FA sensing mechanisms operate in enteroendocrine cells: intracellularly, FA (≥C12) transiently induce Ca2+ release from intracellular Ca2+ stores. However, they also induce sustained Ca2+ entry from the extracellular medium to maintain an elevated [Ca2+]i. |
| Rights: | This research was originally published in the Journal of Biological Chemistry. Author(s). Multiple Fatty Acid Sensing Mechanisms Operate in Enteroendocrine Cells. J. Biol. Chem. 2004; Vol.279: 26082–26089. © the American Society for Biochemistry and Molecular Biology or © the Author(s). | | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/84549 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 比良 徹
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