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Flazin as a Lipid Droplet Regulator against Lipid Disorders
| Title: | Flazin as a Lipid Droplet Regulator against Lipid Disorders |
| Authors: | Wu, Xunzhi Browse this author | | Chen, Zhen Browse this author →KAKEN DB | | Wu, Yue Browse this author | | Chen, Yifan Browse this author | | Jia, Jiaping Browse this author | | Shen, Nianqiu Browse this author | | Chiba, Hitoshi Browse this author →KAKEN DB | | Hui, Shu-Ping Browse this author →KAKEN DB |
| Keywords: | lipid-storage disorders | | metabolic diseases | | diabetic nephropathy | | nutraceuticals | | functional foods | | triglyceride | | lipid metabolism | | lipidomics | | mass spectrometry |
| Issue Date: | 3-Apr-2022 |
| Publisher: | MDPI |
| Journal Title: | Nutrients |
| Volume: | 14 |
| Issue: | 7 |
| Start Page: | 1501 |
| Publisher DOI: | 10.3390/nu14071501 |
| PMID: | 35406114 |
| Abstract: | Lipid disorders are closely related to numerous metabolic diseases, and lipid droplets (LDs) have been considered as a new target for regulating lipid metabolism. Dietary intervention and nutraceuticals provide safe and long-term beneficial effects for treating metabolic diseases. Flazin is a diet-derived bioactive constituent mainly existing in fermented foods, of which the lipid metabolism improvement function has not been studied. In this study, the effect of flazin on lipid regulation at both cell level and organelle level was investigated. Lipidomic profiling showed that flazin significantly decreased cellular triglyceride (TG) by 12.0-22.4% compared with modeling groups and improved the TG and free fatty acid profile. LD staining revealed that flazin efficiently reduced both cellular neutral lipid content by 17.4-53.9% and LD size by 10.0-35.3%. Furthermore, nanoelectrospray ionization mass spectrometry analysis proved that flazin exhibited a preferential suppression of LD TG and regulated LD morphology, including a size decrease and surface property improvement. An evaluation of related gene expression suggested the mechanism to be lipolysis promotion and lipogenesis inhibition. These findings indicated that flazin might be an LD regulator for reversing lipid metabolism disturbance. Moreover, the strategy proposed in this study may contribute to developing other nutraceuticals for treating lipid disorder-related metabolic diseases. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/85171 |
| Appears in Collections: | 保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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