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Comparison of phasing methods for sulfur-SAD using in-house chromium radiation : case studies for standard proteins and 69-kDa protein
| Title: | Comparison of phasing methods for sulfur-SAD using in-house chromium radiation : case studies for standard proteins and 69-kDa protein |
| Authors: | Watanabe, Nobuhisa Browse this author | | Kitago, Yu Browse this author | | Tanaka, Isao Browse this author →KAKEN DB | | Wang, Jia-wei Browse this author | | Gu, Yuan-xin Browse this author | | Zheng, Chao-de Browse this author | | Fan, Hai-fu Browse this author |
| Keywords: | single-wavelength anomalous diffraction | | sulfur SAD | | chromium X-ray radiation |
| Issue Date: | Nov-2005 |
| Publisher: | International Union of Crystallography |
| Journal Title: | Acta Crystallographica section D |
| Volume: | 61 |
| Issue: | 11 |
| Start Page: | 1533 |
| End Page: | 1540 |
| Publisher DOI: | 10.1107/S0907444905028416 |
| PMID: | 16239732 |
| Abstract: | Phasing of the crystal structures of four standard proteins (lysozyme, trypsin, glucose isomerase, and thaumatin) and a novel 69-kDa protein from Thermus thermophilus, TT0570, was performed using the singlewavelength anomalous diffraction of sulfur atoms intrinsically present within the native protein molecules. To utilize the sulfur anomalous diffraction, the data sets were collected using the loop-less data collection method with chromium Kα X-rays of 2.29Å. Three phasing methods, MLPHARE, SHARP, and OASIS-2004, were tested in combination with the DM or SOLOMON density modification method. The results showed that the solvent contents are still an important factor for phasing with the S-SAD method, even when longer wavelength Cr Kα radiation is used. Among the three procedures, the improved direct phasing of OASIS-2004 with its implemented fragment feedback to the direct-method probability calculation gave the best results in determining the initial phases. For all five proteins, almost the entire models could be built automatically. |
| Rights: | Copyright © International Union of Crystallography |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/8533 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡邉 信久
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