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Comparison of phasing methods for sulfur-SAD using in-house chromium radiation : case studies for standard proteins and 69-kDa protein

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タイトル: Comparison of phasing methods for sulfur-SAD using in-house chromium radiation : case studies for standard proteins and 69-kDa protein
著者: Watanabe, Nobuhisa 著作を一覧する
Kitago, Yu 著作を一覧する
Tanaka, Isao 著作を一覧する
Wang, Jia-wei 著作を一覧する
Gu, Yuan-xin 著作を一覧する
Zheng, Chao-de 著作を一覧する
Fan, Hai-fu 著作を一覧する
キーワード: single-wavelength anomalous diffraction
sulfur SAD
chromium X-ray radiation
発行日: 2005年11月
出版者: International Union of Crystallography
誌名: Acta Crystallographica section D
巻: 61
号: 11
開始ページ: 1533
終了ページ: 1540
出版社 DOI: 10.1107/S0907444905028416
抄録: Phasing of the crystal structures of four standard proteins (lysozyme, trypsin, glucose isomerase, and thaumatin) and a novel 69-kDa protein from Thermus thermophilus, TT0570, was performed using the singlewavelength anomalous diffraction of sulfur atoms intrinsically present within the native protein molecules. To utilize the sulfur anomalous diffraction, the data sets were collected using the loop-less data collection method with chromium Kα X-rays of 2.29Å. Three phasing methods, MLPHARE, SHARP, and OASIS-2004, were tested in combination with the DM or SOLOMON density modification method. The results showed that the solvent contents are still an important factor for phasing with the S-SAD method, even when longer wavelength Cr Kα radiation is used. Among the three procedures, the improved direct phasing of OASIS-2004 with its implemented fragment feedback to the direct-method probability calculation gave the best results in determining the initial phases. For all five proteins, almost the entire models could be built automatically.
Rights: Copyright © International Union of Crystallography
関連URI: http://scripts.iucr.org/cgi-bin/paper?dz5050
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/8533
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 渡邉 信久

 

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