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Hokkaido University Collection of Scholarly and Academic Papers >
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Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaC(AR)
| Title: | Versatile aliphatic polyester biosynthesis system for producing random and block copolymers composed of 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates using the sequence-regulating polyhydroxyalkanoate synthase PhaC(AR) |
| Authors: | Satoh, Keigo Browse this author | | Kawakami, Tomoya Browse this author | | Isobe, Nagi Browse this author | | Pasquier, Loic Browse this author | | Tomita, Hiroya Browse this author | | Zinn, Manfred Browse this author | | Matsumoto, Ken'ichiro Browse this author →KAKEN DB |
| Keywords: | PHA synthase | | Block copolymer | | 2-Hydroxybutyrate | | 4-Hydroxy-2-methylbutanoate | | 5-Hydroxypentanoate | | delta-Valerolactone | | epsilon-Caprolactone | | Sequence regulation | | Biodegradable plastic |
| Issue Date: | 14-May-2022 |
| Publisher: | BioMed Central |
| Journal Title: | Microbial Cell Factories |
| Volume: | 21 |
| Issue: | 1 |
| Start Page: | 84 |
| Publisher DOI: | 10.1186/s12934-022-01811-7 |
| Abstract: | Background: Polyhydroxyalkanoates (PHAs) are microbial polyesters synthesized by PHA synthases. Naturally occurring PHA copolymers possess a random monomer sequence. The development of PhaC(AR), a unique sequence-regulating PHA synthase, has enabled the spontaneous biosynthesis of PHA block copolymers. PhaC(AR) synthesizes both a block copolymer poly(2-hydroxybutyrate)-b-poly(3-hydroxybutyrate) [P(2HB)-b-P(3HB)], and a random copolymer, poly(3HB-co-3-hydroxyhexanoate), indicating that the combination of monomers determines the monomer sequence. Therefore, in this study, we explored the substrate scope of PhaC(AR) and the monomer sequences of the resulting copolymers to identify the determinants of the monomer sequence. PhaC(AR) is a class I PHA synthase that is thought to incorporate long-main-chain hydroxyalkanoates (LMC HAs, > C-3 in the main [backbone] chain). Thus, the LMC monomers, 4-hydroxy-2-methylbutyrate (4H2MB), 5-hydroxyvalerate (5HV), and 6-hydroxyhexanoate (6HHx), as well as 2HB, 3HB, and 3-hydroxypropionate (3HP) were tested. Results: Recombinant Escherichia coli harboring PhaC(AR), CoA transferase and CoA ligase genes was used for PHA production. The medium contained the monomer precursors, 2HB, 3HB, 3HP, 4H2MB, 5HV, and 6HHx, either individually or in combination. As a result, homopolymers were obtained only for 3HB and 3HP. Moreover, 3HB and 3HP were randomly copolymerized by PhaC(AR). 3HB-based binary copolymers P(3HB-co-LMC HA)s containing up to 2.9 mol% 4H2MB, 4.8 mol% 5HV, or 1.8 mol% 6HHx were produced. Differential scanning calorimetry analysis of the copolymers indicated that P(3HB-co-LMC HA)s had a random sequence. In contrast, combining 3HP and 2HB induced the synthesis of P(3HP)-b-P(2HB). Similarly, P(2HB) segment-containing block copolymers P(3HB-co-LMC HA)-b-P(2HB)s were synthesized. Binary copolymers of LMC HAs and 2HB were not obtained, indicating that the 3HB or 3HP unit is essential to the polymer synthesis. Conclusion: PhaC(AR) possesses a wide substrate scope towards 2-, 3-, 4-, 5-, and 6-hydroxyalkanoates. 3HB or 3HP units are essential for polymer synthesis using PhaC(AR). The presence of a 2HB monomer is key to synthesizing block copolymers, such as P(3HP)-b-P(2HB) and P(3HB-co-LMC HA)-b-P(2HB)s. The copolymers that did not contain 2HB units had a random sequence. This study's results provide insights into the mechanism of sequence regulation by PhaC(AR) and pave the way for designing PHA block copolymers. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/86112 |
| Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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