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Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN

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Title: Epigenetic regulation of ecotype-specific expression of the heat-activated transposon ONSEN
Authors: Nozawa, Kosuke Browse this author
Masuda, Seiji Browse this author →KAKEN DB
Saze, Hidetoshi Browse this author
Ikeda, Yoko Browse this author
Suzuki, Takamasa Browse this author
Takagi, Hiroki Browse this author
Tanaka, Keisuke Browse this author
Ohama, Naohiko Browse this author
Niu, Xiaoying Browse this author
Kato, Atsushi Browse this author →KAKEN DB
Ito, Hidetaka Browse this author →KAKEN DB
Keywords: transposons
environmental stress
Arabidopsis thaliana
Issue Date: 18-Jul-2022
Publisher: Frontiers Media
Journal Title: Frontiers in Plant Science
Volume: 13
Start Page: 899105
Publisher DOI: 10.3389/fpls.2022.899105
Abstract: Transposable elements are present in a wide variety of organisms; however, our understanding of the diversity of mechanisms involved in their activation is incomplete. In this study, we analyzed the transcriptional activation of the ONSEN retrotransposon, which is activated by high-temperature stress in Arabidopsis thaliana. We found that its transcription is significantly higher in the Japanese ecotype Kyoto. Considering that transposons are epigenetically regulated, DNA methylation levels were analyzed, revealing CHH methylation was reduced in Kyoto compared to the standard ecotype, Col-0. A mutation was also detected in the Kyoto CMT2 gene, encoding a CHH methyltransferase, suggesting that it may be responsible for increased expression of ONSEN. CHH methylation is controlled by histone modifications through a self-reinforcing loop between DNA methyltransferase and histone methyltransferase. Analysis of these modifications revealed that the level of H3K9me2, a repressive histone marker for gene expression, was lower in Kyoto than in Col-0. The level of another repressive histone marker, H3K27me1, was decreased in Kyoto; however, it was not impacted in a Col-0 cmt2 mutant. Therefore, in addition to the CMT2 mutation, other factors may reduce repressive histone modifications in Kyoto.
Type: article
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伊藤 秀臣

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