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Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes

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タイトル: Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes
著者: Shinozuka, H 著作を一覧する
Hisano, H 著作を一覧する
Ponting, R.C. 著作を一覧する
Cogan, N.O.I. 著作を一覧する
Jones, E.S. 著作を一覧する
Forster, J.W. 著作を一覧する
Yamada, T 著作を一覧する
キーワード: Perennial ryegrass
Casein kinase 2 α
Single nucleotide polymorphism
Cleaved amplified polymorphic sequence
Heading date
Conserved synteny
発行日: 2005年12月
出版者: Springer-Verlag GmbH
誌名: TAG Theoretical and Applied Genetics
巻: 112
号: 1
開始ページ: 167
終了ページ: 177
出版社 DOI: 10.1007/s00122-005-0119-8
抄録: The α-subunit of the casein protein kinase CK2 has been implicated in both light-regulated and circadian rhythm-controlled plant gene expression, including control of the flowering time. Two putative CK2α genes of perennial ryegrass (Lolium perenne L.) have been obtained from a cDNA library constructed with mRNA isolated from cold-acclimated crown tissue. The genomic organisation of the two genes was determined by Southern hybridisation analysis. Primer designs to the Lpck2a-1 and Lpck2a-2 cDNA sequences permitted the amplification of genomic products containing large intron sequences. Amplicon sequence analysis detected single nucleotide polymorphisms (SNPs) within the p150/112 reference mapping population. Validated SNPs, within diagnostic restriction enzyme sites, were used to design cleaved amplified polymorphic sequence (CAPS) assays. The Lpck2a-1 CAPS marker was assigned to perennial ryegrass linkage group (LG) 4 and the Lpck2a-2 CAPS marker was assigned to LG2. The location of the Lpck2a-1 gene locus supports the previous conclusion of conserved synteny between perennial ryegrass LG4, the Triticeae homoeologous group 5L chromosomes and the corresponding segment of rice chromosome 3. Allelic variation at the Lpck2a-1 and Lpck2a-2 gene loci was correlated with phenotypic variation for heading date and winter survival, respectively. SNP polymorphism may be used for the further study of the role of CK2α genes in the initiation of reproductive development and winter hardiness in grasses.
Rights: The original publication is available at
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 山田 敏彦


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