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Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes
| Title: | Molecular cloning and genetic mapping of perennial ryegrass casein protein kinase 2 α-subunit genes |
| Authors: | Shinozuka, H. Browse this author | | Hisano, H. Browse this author | | Ponting, R.C. Browse this author | | Cogan, N.O.I. Browse this author | | Jones, E.S. Browse this author | | Forster, J.W. Browse this author | | Yamada, T.7 Browse this author →KAKEN DB |
| Authors(alt): | 山田, 敏彦7 |
| Keywords: | Perennial ryegrass | | Casein kinase 2 α | | Single nucleotide polymorphism | | Cleaved amplified polymorphic sequence | | Heading date | | Conserved synteny |
| Issue Date: | Dec-2005 |
| Publisher: | Springer-Verlag GmbH |
| Journal Title: | TAG Theoretical and Applied Genetics |
| Volume: | 112 |
| Issue: | 1 |
| Start Page: | 167 |
| End Page: | 177 |
| Publisher DOI: | 10.1007/s00122-005-0119-8 |
| PMID: | 16240106 |
| Abstract: | The α-subunit of the casein protein kinase CK2 has been implicated in both light-regulated and circadian rhythm-controlled plant gene expression, including control of the flowering time. Two putative CK2α genes of perennial ryegrass (Lolium perenne L.) have been obtained from a cDNA library constructed with mRNA isolated from cold-acclimated crown tissue. The genomic organisation of the two genes was determined by Southern hybridisation analysis. Primer designs to the Lpck2a-1 and Lpck2a-2 cDNA sequences permitted the amplification of genomic products containing large intron sequences. Amplicon sequence analysis detected single nucleotide polymorphisms (SNPs) within the p150/112 reference mapping population. Validated SNPs, within diagnostic restriction enzyme sites, were used to design cleaved amplified polymorphic sequence (CAPS) assays. The Lpck2a-1 CAPS marker was assigned to perennial ryegrass linkage group (LG) 4 and the Lpck2a-2 CAPS marker was assigned to LG2. The location of the Lpck2a-1 gene locus supports the previous conclusion of conserved synteny between perennial ryegrass LG4, the Triticeae homoeologous group 5L chromosomes and the corresponding segment of rice chromosome 3. Allelic variation at the Lpck2a-1 and Lpck2a-2 gene loci was correlated with phenotypic variation for heading date and winter survival, respectively. SNP polymorphism may be used for the further study of the role of CK2α genes in the initiation of reproductive development and winter hardiness in grasses. |
| Rights: | The original publication is available at www.springerlink.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/864 |
| Appears in Collections: | 北方生物圏フィールド科学センター (Field Science Center for Northern Biosphere) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 山田 敏彦
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