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A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields
Title: | A method for the generation of pseudovirus particles bearing SARS coronavirus spike protein in high yields |
Authors: | Fujioka, Yoichiro Browse this author →KAKEN DB | Kashiwagi, Sayaka Browse this author | Yoshida, Aiko Browse this author | Satoh, Aya O. Browse this author | Fujioka, Mari Browse this author | Amano, Maho Browse this author | Yamauchi, Yohei Browse this author | Ohba, Yusuke Browse this author →KAKEN DB |
Keywords: | severe acute respiratory syndrome coronavirus (SARS-CoV) | SARS-CoV-2 | pseudovirus | vesicular stomatitis virus (VSV) | spike protein |
Issue Date: | 28-Apr-2022 |
Publisher: | 一般社団法人 日本細胞生物学会(Japan Society for Cell Biology) |
Journal Title: | Cell structure and function |
Volume: | 47 |
Issue: | 1 |
Start Page: | 43 |
End Page: | 53 |
Publisher DOI: | 10.1247/csf.21047 |
Abstract: | The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has threatened human health and the global economy. Development of additional vaccines and therapeutics is urgently required, but such development with live virus must be conducted with biosafety level 3 confinement. Pseudotyped viruses have been widely adopted for studies of virus entry and pharmaceutical development to overcome this restriction. Here we describe a modified protocol to generate vesicular stomatitis virus (VSV) pseudotyped with SARS-CoV or SARSCoV-2 spike protein in high yield. We found that a large proportion of pseudovirions produced with the conventional transient expression system lacked coronavirus spike protein at their surface as a result of inhibition of parental VSV infection by overexpression of this protein. Establishment of stable cell lines with an optimal expression level of coronavirus spike protein allowed the efficient production of progeny pseudoviruses decorated with spike protein. This improved VSV pseudovirus production method should facilitate studies of coronavirus entry and development of antiviral agents. |
Type: | article |
URI: | http://hdl.handle.net/2115/86796 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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