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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
北海道大学大学院農学研究院邦文紀要 = Memoirs of the Research Faculty of Agriculture, Hokkaido University >
Vol.39 >
体内および体外に由来するマウス胚盤胞期胚のミトコンドリア微細構造における相違
| Title: | 体内および体外に由来するマウス胚盤胞期胚のミトコンドリア微細構造における相違 |
| Other Titles: | Differences in Mitochondrial Ultrastructure between In Vivo and In Vitro Mouse Blastocysts |
| Authors: | 林, 芳弘1 Browse this author | | 江連, 泰知2 Browse this author | | 唄, 花子3 Browse this author →KAKEN DB | | 高橋, 昌志4 Browse this author →KAKEN DB | | 川原, 学5 Browse this author →KAKEN DB |
| Authors(alt): | Hayashi, Yoshihiro1 | | Edure, Taichi2 | | Bai, Hanako3 | | Takahashi, Masashi4 | | Kawahara, Manabu5 |
| Issue Date: | 13-Feb-2023 |
| Publisher: | 北海道大学大学院農学研究院 |
| Journal Title: | 北海道大学大学院農学研究院邦文紀要 |
| Journal Title(alt): | Memories of the Research Faculty of Agriculture, Hokkaido University |
| Volume: | 39 |
| Start Page: | 1 |
| End Page: | 6 |
| Abstract: | Embryo transfer is a widespread assisted reproductive technique for the production of livestock. In general, the blastocyst-stage embryos used for transfer are produced in vivo or in vitro. However, little is known about differences in mitochondrial ultrastructure between in vivo and in vitro blastocysts in mammals. This study aimed to compare the mitochondrial ultrastructure between in vivo and in vitro mouse blastocysts using transmission electron microscopy (TEM). TEM analyses were performed using more than 1,000 mitochondria from blastocyst embryos. No significant difference was observed in mitochondrial roundness. However, the average major axes (nm) and areas (nm2) were significantly greater in the in vitro embryos than in the in vivo embryos (p＜0.01). Furthermore, the average electron density was significantly decreased in the in vitro embryos compared to the in vivo embryos (p＜0.01). Taken together, we conclude that mitochondria in in vitro blastocysts show an increase in size and a decrease in electron density compared with those in in vivo blastocysts. This implies that mitochondrial functions are deteriorated in in vitro blastocysts compared to in vivo blastocysts. |
| Type: | bulletin (article) |
| URI: | http://hdl.handle.net/2115/88075 |
| Appears in Collections: | 北海道大学大学院農学研究院邦文紀要 = Memoirs of the Research Faculty of Agriculture, Hokkaido University > Vol.39
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