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W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori

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Title: W-derived BAC probes as a new tool for identification of the W chromosome and its aberrations in Bombyx mori
Authors: Sahara, Ken1 Browse this author →KAKEN DB
Yoshido, Atsuo Browse this author
Kawamura, Naoko Browse this author
Ohnuma, Akio Browse this author
Abe, Hiroaki Browse this author
Mita, Kazuei Browse this author
Oshiki, Toshikazu Browse this author
Shimada, Toru Browse this author
Asano, Shin-ichiro Browse this author
Bando, Hisanori Browse this author →KAKEN DB
Yasukochi, Yuji Browse this author
Authors(alt): 佐原, 健1
Keywords: comparative genomic hybridization
ephestia-kuehniella lapidoptera
domesticated silkworm
translocation strains
linkage map
Issue Date: 24-Jun-2003
Publisher: Springer-Verlag GmbH
Journal Title: Chromosoma
Volume: 112
Issue: 1
Start Page: 48
End Page: 55
Publisher DOI: 10.1007/s00412-003-0245-5
PMID: 12827381
Abstract: We isolated four W chromosome-derived bacterial artificial chromosome (W-BAC) clones from Bombyx mori BAC libraries by the polymerase chain reaction and used them as probes for fluorescence in situ hybridization (FISH) on chromosome preparations from B. mori females. All four W-BAC probes surprisingly highlighted the whole wild-type W sex chromosome and also identified the entire original W-chromosomal region in W chromosome-autosome translocation mutants. This is the first successful identification of a single chromosome by means of BAC-FISH in species with holokinetic chromosomes. Genomic in situ hybridization (GISH) by using female-derived genomic probes highlighted the W chromosome in a similar chromosome-painting manner. Besides the W, hybridization signals of W-BAC probes also occurred in telomeric and/or subtelomeric regions of the autosomes. These signals coincided well with those of female genomic probes except one additional GISH signal that was observed in a large heterochromatin block of one autosome pair. Our results support the opinion that the B. mori W chromosome accumulated transposable elements and other repetitive sequences that also occur, but scattered, elsewhere in the respective genome. Edited by: E.R. Schmidt
Rights: The original publication is available at
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐原 健

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