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Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors
Title: | Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors |
Authors: | Omoteyama, Kazuki Browse this author | Ikeda, Hiromi Browse this author | Imaki, Junko Browse this author | Sakai, Masaharu4 Browse this author →KAKEN DB |
Authors(alt): | 酒井, 正春4 |
Keywords: | c-Maf | Lc-Maf | CTGF | Hypertrophic chondrocyte | Differentiation | Transcription control |
Issue Date: | 5-Dec-2005 |
Publisher: | Elsevier Inc. |
Journal Title: | Biochemical and Biophysical Research Communications |
Volume: | 339 |
Issue: | 4 |
Start Page: | 1089 |
End Page: | 1097 |
Publisher DOI: | 10.1016/j.bbrc.2005.11.119 |
PMID: | 16343439 |
Abstract: | The Maf family of transcription factors is expressed during development of various organs and tissues, and is involved in a variety of developmental and cellular differentiation processes. We previously found that c-maf and mafB are strongly expressed in hypertrophic chondrocytes during cartilage development. Connective tissue growth factor (CTGF) is also expressed in hypertrophic chondrocytes. Adenovirus mediated introduction of c-maf gene into the mouse fibroblast cell line C3H10T1/2 strongly induced CTGF expression. CTGF can be induced by TGF-β via the SMAD pathway; however, the c-Maf could not induce TGF-β, nor could TGF-β induce the c-Maf, suggesting that activation of CTGF by Maf is TGF-β independent. Reporter transfection analysis using C3H10T1/2 cells shows that c-Maf stimulates a CTGF reporter gene. Lc-Maf, a splice variant of c-Maf containing an extra 10 amino acids in the carboxyl terminus, was a stronger inducer of the CTGF reporter gene than c-Maf. Chromatin immunoprecipitation analysis showed that c-Maf binds to the promoter region of the CTGF gene, indicating that Maf directly activates the CTGF gene. Gel mobility shift assays indicated that c-Maf binds to the region near the transcription initiation site of CTGF gene. Taken together, these data indicate that the CTGF gene is a target of c-Maf and Lc-Maf in cartilage development. |
Relation: | http://www.sciencedirect.com/science/journal/0006291X |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/989 |
Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 酒井 正春
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