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Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors

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Title: Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors
Authors: Omoteyama, Kazuki Browse this author
Ikeda, Hiromi Browse this author
Imaki, Junko Browse this author
Sakai, Masaharu4 Browse this author →KAKEN DB
Authors(alt): 酒井, 正春4
Keywords: c-Maf
Hypertrophic chondrocyte
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Issue Date: 5-Dec-2005
Publisher: Elsevier Inc.
Journal Title: Biochemical and Biophysical Research Communications
Volume: 339
Issue: 4
Start Page: 1089
End Page: 1097
Publisher DOI: 10.1016/j.bbrc.2005.11.119
PMID: 16343439
Abstract: The Maf family of transcription factors is expressed during development of various organs and tissues, and is involved in a variety of developmental and cellular differentiation processes. We previously found that c-maf and mafB are strongly expressed in hypertrophic chondrocytes during cartilage development. Connective tissue growth factor (CTGF) is also expressed in hypertrophic chondrocytes. Adenovirus mediated introduction of c-maf gene into the mouse fibroblast cell line C3H10T1/2 strongly induced CTGF expression. CTGF can be induced by TGF-β via the SMAD pathway; however, the c-Maf could not induce TGF-β, nor could TGF-β induce the c-Maf, suggesting that activation of CTGF by Maf is TGF-β independent. Reporter transfection analysis using C3H10T1/2 cells shows that c-Maf stimulates a CTGF reporter gene. Lc-Maf, a splice variant of c-Maf containing an extra 10 amino acids in the carboxyl terminus, was a stronger inducer of the CTGF reporter gene than c-Maf. Chromatin immunoprecipitation analysis showed that c-Maf binds to the promoter region of the CTGF gene, indicating that Maf directly activates the CTGF gene. Gel mobility shift assays indicated that c-Maf binds to the region near the transcription initiation site of CTGF gene. Taken together, these data indicate that the CTGF gene is a target of c-Maf and Lc-Maf in cartilage development.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 酒井 正春

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