Permalink : http://hdl.handle.net/2115/84971

JaLCDOI : 10.14943/EJFR.22.12

KEYWORDS : haskap;imaging mass spectrometry;interspecific hybridization;LC/MS;Lonicera caerulea;Lonicera gracilipes

There are many useful genetic resources as berry crop candidates in Hokkaido, Japan. Representative plant genetic resources are haskap ( Lonicera caerulea; Caprifoliaceae), Rubus idaeus (Rosaceae), Actinidia arguta (Actinidiaceae), and so on. Among them, this manuscript focuses on haskap, showing attractive features for commercial production in the Hokkaido regions. In this manuscript, characterization of the wild genetic resources is described. Both diploid (2n = 2x = 18) and tetraploid (2n = 4x = 36) were found in Hokkaido. Our study showed that tetraploid haskap populations are widely distributed in Hokkaido, while diploid populations are distributed only in eastern Hokkaido. The factors affecting this distribution pattern are now analyzed. As an attempt to improve haskap accessions, interspecific hybridization has been applied between haskap and Miyama -uguiskagura (Lonicera gracilipes), belonging to the same genus as haskap. The interspecific hybrids were fertile and could produce fruits. To characterize the fruit contents of the parental species and interspecific hybrids, liquid chromatography/tandem mass spectrometry (LC/MS) and imaging mass spectrometry were used for detailed analyses.