Japanese Journal of Veterinary Research;Volume 2, Number 1

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GUANOFURACIN MEDIA FOR ISOLATION OF L. MONOCYTOGENES AND ITS PRACTICAL APPLICATION

SHIMIZU, Kiheiji;OTSUKA, Giichi;OKA, Motoi

Permalink : http://hdl.handle.net/2115/1650
JaLCDOI : 10.14943/jjvr.2.1.1

Abstract

In the present report, it is the selective and enrichment media for Listeria monocytogenes and their practical application that are mainly described. 1. One per cent glucose nutrient broth with 1 : 12,000 concentration of guanofuracin will be recommened as an enrichment medium, and 1% glucose nutrient agar with 5% sheep-blood and 1 : 10,000 concentration of guanofuracin as a selective solid medium. In the latter medium, addition of 0.01% potassium tellurite is beneficial for the differentiation by the coloring of the colony. 2. By using these selective media, Listeria monocytogenes was detected in one nasal swab from a total 183 sheep. From the viewpoint of epidemiology of this disease, this finding will suggest the existence of a healthy carrier of the organism.

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