Japanese Journal of Veterinary Research;Volume 3, Number 2

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BIOCHEMICAL STUDIES ON THE INCREASE AND FORMATION OF ANTIBODIES WITH CHYMOPAPAIN IN VITRO : III. STUDIES ON THE INCREASE OF DIPHTHERIAL ANTITOXIC TITERS FROM PRELIMINARILY DENATURATED ANTITOXIC GLOBULINS

ITO, Tokiya

Permalink : http://hdl.handle.net/2115/1671
JaLCDOI : 10.14943/jjvr.3.2.105

Abstract

The present data indicate that the pre-denaturated diphtherial antitoxins produced by acid-heating, by alkali-heating, by urea or by sodium salicylate addition, from the large incomplete antitoxic molecules, those produced in a horse at the initial stages of diphtherial immunization, are not converted newly into small and complete antitoxic molecules in the incubating procedures with oxidized chymopapain and diphtherial toxin, in which those produced with pepsin-predigestion were converted into. In these studies, the fact was found that to increase the diphtherial antitoxic titers of immune equine serum globulins, which obtained from horse at the initial stages of immunization, with oxidized chymopapain and diphtherial toxin under suitable definite conditions of the incubation in vitro, the pre-digestion of large incomplete antitoxic globulins with pepsin is particularily necessary.

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