Permalink : http://hdl.handle.net/2115/32486

KEYWORDS : Regiospecific analysis;High-performance thin-layer chromatography;Triacylglycerol;Fatty acid;Fish oil;Seal oil

This paper presents a smaller-sized procedure for regiospecific analysis of triacylglycerols (TAG) using boric acid high-performance thin-layer chromatography (HPTLC). Cod liver/mackerel, bonito head, and seal oils TAG (2mg) were partially hydrolyzed by ethyl magnesium bromide, and resulting 1(3)-and 2-monoacylglycerols (MAG) were isolated by the HPTLC. Fatty acids of the 1(3)-and 2-MAG were analyzed by gas-liquid chromatography (GLC). Positional distributions of fatty acids in TAG observed for the three marine oils were similar to those previously determined by the procedure requiring 100mg of starting TAG. The new procedure appeared to be usable for regiospecific analysis of a few milligrams of marine oils TAG.