Japanese Journal of Veterinary Research;Volume 11, Number 4

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ROLE OF PROTEOLYTIC ENZYME IN TOXIN PRODUCTION BY CLOSTRIDIUM BOTULINUM TYPE A

INUKAI, Yoshikazu

Permalink : http://hdl.handle.net/2115/1785
JaLCDOI : 10.14943/jjvr.11.4.143

Abstract

1. Three proteolytic enzymes A, B, and C were isolated from a culture filtrate of Cl. botulinum type A No. 38 by chromatography using DEAE-cellulose. 2. Optimum pH for proteolytic activity of these enzymes A, B, and C were respectively 8.5,7.5 and 6.0 or below. 3. Of these enzymes only C was able to enhance the toxicity of the protoxin preparation. 4. Enzymes A, B, and C acted more or less destructively on type A toxin at alkaline pH level. 5. It was suggested indirectly that a fragmentation of the toxin might occur after activation in the culture. 6. On the basis of the results obtained, a process of toxin production by type A organisms was discussed.

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