Japanese Journal of Veterinary Research;Volume 15, Number 2

FONT SIZE:  S M L

STUDIES ON ECHINOCOCCOSIS XIX : HEAT STABLE ANTIGEN IN CYSTIC FLUID OF ECHINOCOCCUS MULTILOCULARIS

ORIHARA, Miyoji

Permalink : http://hdl.handle.net/2115/1870
JaLCDOI : 10.14943/jjvr.15.2.85

Abstract

For inactivation of antigenicity of host components in cystic fluid of E. multilocularis, heat treatment experiments were conducted. Fractions which are reactive to serum from infected mice with E. multilocularis were collected by gel filtration of Sephadex G 75,and were heated at 100℃ for 15 minutes. For a comparative study, mild heating was also performed. For serological tests, the CF test and gel diffusion test were used. Experimental results obtained may be summarized as follows : 1) Reactive components of host origin in cystic fluid are partially lost at 70℃ for 20 minutes and are almost destroyed at 100℃ for 15 minutes. 2) In the gel diffusion test, most antigens originated from the parasite in cystic fluid are stable at 100℃ for 15 minutes in test with serum from mice with E. multilocularis, and these precipitates are stained strongly with PAS. So, polysaccharides seem to take a part in these precipitates. In antigens originated from the parasite, heat labile antigens are also present while it is not often reactive in test with sera from mice. 3) In the CF test, heated fractionated Emc titer is low, in comparison with raw fractionated Emc in test with sera from human beings and infected mice.

FULL TEXT:PDF