Japanese Journal of Veterinary Research;Volume 15, Number 3

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NUTRITIONAL REQUIREMENTS OF CORYNEBACTERIUM RENALE

HIRAI, Katsuya;YANAGAWA, Ryo

Permalink : http://hdl.handle.net/2115/1884
JaLCDOI : 10.14943/jjvr.15.3.121

Abstract

The growth factor and amino acid requirements of Corynebacterium renale were investigated with special reference to the 3 types of this species reported by YANAGAWA et al. (1967). The results are summarized as follows. 1) Strains of type I required thiamine, biotin and pantothenic acid for maximum growth. These vitamins acted as growth stimulatory factors. Strains of type II required biotin, nicotinic acid and p-aminobenzoic acid as essential growth factors. Thiamine and pantothenic acid acted as growth stimulants. Strains of type III which was most exacting, required thiamine, biotin, nicotinic acid, pantothenic acid and pyridoxine as essential growth factors. Without even one of these vitamins type III strains did not grow. Pyridoxine was required by type III but not by type II while p-aminobenzoic acid was essential for type II but not for type III. 2) Sodium bicarbonate stimulated the growth of all 3 types of C. renale. 3) Ammonium sulfate was necessary for maximum growth of each type. 4) The essential amino acid common to the 3 types of C. renale included glutamic acid, valine and isoleucine, Type I required only these 3 amino acids. Type II required the 3 amino acids essentially ; the growth was accelerated with the addition of methionine, phenylalanine and histidine. Growth of type III was most exacting. In addition to the common 3 amino acids, it required tryptophane essentially. Methionine, phenylalanine and arginine were also needed for maximum growth. Thus, in complexity, the requirements of both growth factors and amino acids was as follows : types III, II and I. 5) Growth inhibition by cystine was particularly noticeable in type I. 6) There was discussion on the comparison of the nutritional requirements of C. diphtheriae, C. sepedonicum and C. renale.

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