Japanese Journal of Veterinary Research;Volume 16, Number 4

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CHARACTERISTICS OF CORYNEBACTERIUM RENALE PHAGE

YANAGAWA, Ryo;SHINAGAWA, Morikazu

Permalink : http://hdl.handle.net/2115/1915
JaLCDOI : 10.14943/jjvr.16.4.137

Abstract

Methods of characterizing phage have been applied to the phages obtained from 10 strains of Corynebacterium renale, isolated in Japan. These phages are identical morphologically, serologically and in host range. They have a polygonal head 50 mμ in diameter and a tail 260 mμ in length and 10 mμ wide. The tail is exhibiting a capsomere structure. The tail tips have a sixfold star-shaped assembly and very fine fibers are developed from the tips. Phage titers of 10^9~10^<10> plaque-forming units per ml were finally obtained after several propagations with C. renale No. 71 by the soft agar method of increasing the volume of the propagating bacteria. The following data were also obtained from a representative phage RP 6 and the propagating bacteria. Some loss in activity resulted from heating at 50℃, and in 3% chloroform. All activity was lost by heating at 55℃ for 30 min. It had a slow adsorption rate (K=5.5×10^<-11> ml/min) with a latent period of 50 min and a burst size of 16 particles. Its nucleic acid was a double-stranded DNA.

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