Japanese Journal of Veterinary Research;Volume 17, Number 1-2

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INTERFERON-LIKE INHIBITOR DETECTED IN CULTURE FLUIDS OF NORMAL HELA CELLS

KINJO, Toshio;YANAGAWA, Ryo

Permalink : http://hdl.handle.net/2115/1922
JaLCDOI : 10.14943/jjvr.17.1-2.43

Abstract

Normal HeLa cells produced an interferon-like inhibitor against ICH virus and this was demonstrated in cultures of normal HeLa cells when tested in DKC. No interferon was detected in cultures of HeLa cells infected with ICH virus. This interferon-like inhibitor was not sedimented by centrifugation at 36,000 rpm for 90 min and was shown to be non-dialyzable against a pH 2 solution. Its activity was not inactivated by heating at 58℃ for 30 min or by treatment with anti-ICH virus immune serum. Neither DNase nor RNase had any effect on the activity of the inhibitor. However, its activity was largely lost after treatment with trypsin. These properties of the interferon-like inhibitor were similar to those of interferon. However the origin of the interferon-like inhibitor was completely distinct from that of interferon because the former was produced in the cultures of normal HeLa cells.

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