Japanese Journal of Veterinary Research;Volume 18, Number 1

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VIRUS PARTICLES (LEUKOSIS/SARCOMA GROUP LIKE VIRUSES) IN THE PERIPHERAL NERVE AND THE SKELETAL MUSCLE TISSUES WITH MAREK'S DISEASE (FOWL PARALYSIS)

OKADA, Kosuke;FUJIMOTO, Yutaka

Permalink : http://hdl.handle.net/2115/1942
JaLCDOI : 10.14943/jjvr.18.1.21

Abstract

Two chickens with Marek's disease (MD)(Fowl paralysis) and one apparently normal chicken, which were diagnosed by clinical features and histopathology, were observed by electron microscopy. One bird with lymphoid leukosis was also examined for comparison. Association and multiplication of virus particles (VP) in the peripheral nerves and the skeletal muscles accompanied by degenerative changes were unexpectedly observed. Mature VP measuring from 80 to 100 mμ in diameter, covered with double membranes (inner and outer), had an electron opaque central nucleoid measuring 40 to 55 mμ and had numerous peripheral knobs on the outside of the particles. In the cytoplasm, there was viroplasm consisting of immature small VP (virospheres, from 70 to 80 mμ) surrounded by granules of the size of ribosomes (virosomes), viral matrix consisting of aggregates of electron opaque granules, and gray bodies, involving a few VP, surrounded by a single membrane. These VP matured by budding from the cytoplasmic membrane. Numerous VP were found in the lumen and intercellular spaces of the capillaries, in the endothelial cells, in the pericytes, in the intracytoplasmic vesicles of emigrating macrophages, in the axon, in the basement membrane of the unmyelinated nerve fibers, in the nerve cells, in the intercellular spaces of satellite cells, and in the sarcoplasm and basement membranes of the skeletal muscles. These VP at present under investigation seemed to be similar to those of the leukosis/sarcoma group of viruses, because of their morphology and mode of multication.

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