Japanese Journal of Veterinary Research;Volume 56, Number 2

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Expression of α-hemoglobin stabilizing protein and cellular prion protein in a subclone of murine erythroleukemia cell line MEL

Otsuka, Yayoi;Ito, Daisuke;Katsuoka, Kayoko;Arashiki, Nobuto;Komatsu, Tomohiko;Inaba, Mutsumi

Permalink : http://hdl.handle.net/2115/34675
JaLCDOI : 10.14943/jjvr.56.2.75
KEYWORDS : α-hemoglobin stabilizing protein;erythroid;murine erythroleukemia;prion protein

Abstract

α-Hemoglobin stabilizing protein (AHSP) functions as the erythroid-specific molecular chaperon for α-globin. AHSP gene expression has been reported to be downregulated in hematopoietic tissues of animals suffering from prion diseases though the mechanism remains to be clarified. Herein, we demonstrate that MELhipod8 cells, a subclone of murine erythroleukemia (MEL) cells, have prion protein (PrPC) on the cell surface and have highly inducible expression of the AHSP and α- and β-globin genes, resembling the expression pattern of the PrP and AHSP genes in bipotential erythroid- and megakaryocyte-lineage cells followed by erythroid differentiation in normal erythropoiesis. Moreover, MELhipod8 cells exhibit greater effective erythroid differentiation with a population of hemoglobinized normoblast-like cells than that observed for the parental MEL cells. These findings suggest that MELhipod8 cells could provide a mechanism for downregulation of the AHSP gene in prion diseases.

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