Japanese Journal of Veterinary Research;Volume 18, Number 4

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HEMATOLOGICAL OBSERVATIONS OF LEUKOCYTES COLLECTED BY WATER METHOD FROM BOVINE PERIPHERAL BLOOD

ODAJIMA, Mikiyo;SONODA, Mitsuo

Permalink : http://hdl.handle.net/2115/1967
JaLCDOI : 10.14943/jjvr.18.4.173

Abstract

An attempt was made to collect a large number of leukocytes without red cells from bovine peripheral blood by the use of the water method improved by the authors, then they were examined hematologically. 1) In the collection of leukocytes by the water method, it was found that using blood and water, in the ratio of 1 to 1.5,with the restoration of isotonicity after 20 seconds was the most favorable condition for bovine blood. 2) In the light microscopy of the collected leukocytes smears stained with Giemsa stain, a significant decrease of normal cells and an increase of destructed cells was observed in comparison with those of the untreated control blood, respectively, but in so-called ghost cells, no significant changes were observed. 3) A significant increase of so-called dead cells was observed in the collected cells in comparison with those of the untreated control blood. 4) By the treatment with water, the phagocytic activities of carbon particles were decreased significantly in monocytes and eosinophils, but in neutrophils no significant changes were observed in comparison with those of the untreated control blood. 5) In the electron microscopy of the collected leukocytes, the defects of the fine structure of the cells were not so marked that almost all the collected cells distinguished their kind of cells by the characteristics of their fine structures. 6) In the differential count of the collected leukocytes, a significant increase of neutrophils and a decrease of lymphocytes was observed, but no significant changes were observed in monocytes, eosinophils and basophils in comparison with the untreated control blood.

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