北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University;第58巻 第3号

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エゾバフンウニ Strongylocentrotus intermedius 消化管からタンパク質分解酵素（スブチラーゼ）の精製

浦, 和寛;田中, 恵梨;東藤, 孝;後藤, 孝弘;清水, 幹博;尾島, 孝男;都木, 靖彰

Permalink : http://hdl.handle.net/2115/36084
KEYWORDS : Strongylocentortus intermedius;Protease;Digest organ

Abstract

We purified a subtilase from digestive system of short spined sea urchin Strongylocentortus intermedius by a combination of ion-exchange chromatography and gel filtration. The molecular weight of purified subtilase on SDS-PAGE under both reducing and nonreducing conditions was 35,000. Antiserum against subtilase specifically immunostained its antigen. By Western blot analysis, immunoreactive with this antibody were observed in stomach and intestine.

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