Japanese Journal of Veterinary Research;Volume 26, Number 3-4

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ANTI-TUMOR IMMUNITY AGAINST MAREK'S DISEASE-DERIVED LYMPHOBLASTOID CELL LINE (MSB-1)

SUGIMOTO, Chihiro;KODAMA, Hiroshi;MIKAMI, Takeshi

Permalink : http://hdl.handle.net/2115/2150
JaLCDOI : 10.14943/jjvr.26.3-4.57

Abstract

Cytotoxic effects of peripheral blood lymphocytes (PBL) and immune sera from chickens infected with Marek's disease virus (MDV) or vaccinated with herpesvirus of turkey (HVT) against Marek's disease lymphoblastoid cell line (MSB-1) cells were studied. Cellular immunity was assayed using lymphocytes collected 60 to 180 days postinfection. Lymphocyte cytotoxicity tests revealed that the PBL of MDV infected chickens had cytotoxic effects against MSB-1 but those of HVT-vaccinated chickens did not. In complement-dependent antibody cytotoxicity (CDAC) and antibody-dependent cell-mediated cytotoxicity (ADCC) tests, no cytotoxic activity of sera from MDV- or HVT-infected chickens was demonstrated. However, after the sera of chickens immunized with inactivated MSB-1 cells were extensively absorbed with chicken red blood cells, these sera had cytotoxic effects against MSB-1 cells in CDAC and ADCC tests.

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