Japanese Journal of Veterinary Research;Volume 27, Number 3-4

FONT SIZE:  S M L

SOME ASPECTS OF BOVINE OVA CULTURE IN VITRO

KANAGAWA, Hiroshi

Permalink : http://hdl.handle.net/2115/2175
JaLCDOI : 10.14943/jjvr.27.3-4.49

Abstract

One hundred and thirty-three fertilized ova were grouped into three categories ; normal, young and degenerated on the 5th day after insemination. A simple culture technique of a stoppered test tube with Brinster's medium and a gas mixture was applied. Thirty-eight out of 46 normal ova (82.6%) were developed into the blastocysts. The young and degenerated ova showed very poor development. Ten normal ova were kept for 2〜4 hours at room temperature before culture and 56 normal ova were exposed under microscopic light for 30 seconds or 30 minutes before culture. Nine ova from the former group and 46 ova from the latter group were developed into the blastocysts, respectively.

FULL TEXT:PDF