Japanese Journal of Veterinary Research;Volume 28, Number 3

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COMPARISON OF MEMBRANE IMMUNOFLUORESCENCE AND CYTOTOXICITY TESTS FOR DETECTION OF CELL SURFACE ANTIGENS OF MAREK'S DISEASE LYMPHOMA-DERIVED CELL LINE

SUZUKI, Kazuo;MIKAMI, Takeshi;KODAMA, Hiroshi;ONUMA, Misao;IZAWA, Hisao

Permalink : http://hdl.handle.net/2115/2198
JaLCDOI : 10.14943/jjvr.28.3.67

Abstract

The reactivity of an anti-MDCC-MSB 1 rabbit serum to Marek's disease tumor-associated surface antigen (MATSA) of a Marek's disease lymphomaderived cell line (MDCC-MSB 1) was compared by membrane immunofluorescence and cytotoxicity tests. Following results were obtained by both tests : 1) the similar reactivity of the antiserum in titrations, 2) the similar blocking activity patterns of the soluble antigens from MDCC-MSB 1 cells to the antiserum, and 3) the parallisms between the cytotoxic index and the frequency of positive cells for MATSA of the MDCC-MSB 1 cells treated with proteolytic enzymes. From these results, both tests appeared to be detecting a similar, if not identical, antigen. The cytotoxicity test was slightly more sensitive than the membrane immunofluorescence test.

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