Japanese Journal of Veterinary Research;Volume 28, Number 4

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A CHROMOSOMAL ANALYSIS BASED ON THE G AND C BAND STAINING TECHNIQUES OF THE BUFFALO (BUBALUS BUBALIS)

MIYAKE, Yoh-Ichi;KANAGAWA, Hiroshi;ISHIKAWA, Tsune

Permalink : http://hdl.handle.net/2115/2205
JaLCDOI : 10.14943/jjvr.28.4.122

Abstract

A chromosomal analysis of 3 swamp buffaloes (2 females & 1 male) was carried out by G and C band pattern staining techniques. The diploid number of the swamp buffalo was 48, and the karyotype consisted of 10 meta- or submetacentric chromosomes and 38 acrocentric chromosomes. The X chromosome was the largest acrocentric and the Y chromosome was the smaller acrocentric. Chromosomal pairing and identification were made possible by the distinct G band patterns, which showed clearly that the Y chromosome was the second smallest acrocentric. While the C band technique stained the centromeric region of the acrocentric chromosomes, it failed to stain that of the meta- or submetacentric chromosomes. The X chromosome had the broadest C band in the centromeric region, and a greater part of the Y chromosomal arm was stained darkly by the C band technique.

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