Japanese Journal of Veterinary Research;Volume 28, Number 4

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INFLUENCE OF TEMPERATURE ON THE ANTIGENICITY OF SCHISTOSOMA JAPONICUM LYOPHILIZED EGGS FOR CIRCUMOVAL PRECIPITIN TEST (COPT)

KAMIYA, Haruo

Permalink : http://hdl.handle.net/2115/2208
JaLCDOI : 10.14943/jjvr.28.4.149

Abstract

The influence of temperature on the antigenicity of lyophilized COPT eggs for the diagnosis of schistosomiasis japonica was investigated. Although alterations in temperature caused a gradual decline of antigenicity, stable temperatures, which ranged from 4℃ to 60℃ for 8 month preservation of the eggs, did not so much affect the antigenicity. A little decline of antigenicity was observed by incubation at temperature as high as 70℃ to 120℃ for 1 hour. The eggs incubated at 120℃ for 24 hours and at 150℃ and 200℃ for 1 hour showed a marked decrease in antigenicity. No precipitin with eggs incubated at 200℃ for 6 hours was observed. These results demonstrated that the egg antigens involved in COPT have a heat stable nature. The COPT using lyophilized eggs for the diagnosis of schistosomiasis japonica is strongly recommended in field surveys of the endemic tropics since the egg antigen for COPT is sensitive, specific, and stable in nature and the technique is simple.

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