Japanese Journal of Veterinary Research;Volume 31, Number 3-4

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ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) AS A DIAGNOSTIC TOOL FOR GUATEMALAN ONCHOCERCIASIS USING A BOVINE FILARIA (ONCHOCERCA GUTTUROSA) ANTIGEN AND BLOOD SAMPLES COLLECTED ON FILTER PAPER

ITO, Mamoru;LUJAN-T., Aracely;FUKUMOTO, Shin-ichiro;KAMIYA, Masao

Permalink : http://hdl.handle.net/2115/2302
JaLCDOI : 10.14943/jjvr.31.3-4.141
KEYWORDS : ELISA;Onchocerca gutturosa;Onchocerca volvulus;filaria;Guatemala

Abstract

An ELISA for human onchocerciasis performed in Guatemala with a crude PBS extract of Onchocerca gutturosa and blood samples collected on filter paper showed a high sensitivity and specificity. A correlation between ELISA and IHA was obtained, and the former proved to be more sensitive than the latter. The ELISA values of blood samples collected on filter paper and of sera obtained from the same patient showed a high correlation. The linear correlation was also observed in ELISA values using two types of antigens, i. e., O.gutturosa and O.volvulus. 388 inhabitants of five coffee plantations were examined by the ELISA using O.gutturosa antigen. The results showed a positive reaction in 40(97.6%) of the 41 inhabitants with microfilariae(Mf)-positive/nodule-positive, and in 76(91.6%) out of 83 with Mf-positive/nodule-negative, but in only 17(21.8%) out of 78 with Mf-negative/nodule-negative in an endemic area, and 1(1.1%) out of 90 in a non-endemic area. Using a combination of O.gutturosa obtained from cattle and blood samples collected on filter paper, ELISA was found to be applicable for the immunodiagnosis of human onchocerciasis in Guatemala.

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