Japanese Journal of Veterinary Research;Volume 32, Number 1

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CIRCUMOVAL AND CIRCUMLARVAL PRECIPITATE REACTIONS OF ANGIOSTRONGYLUS CANTONENSIS

OKU, Yuzaburo;KAMIYA, Masao

Permalink : http://hdl.handle.net/2115/2306
KEYWORDS : Angiostrongylus cantonensis;Angiostrongylus costaricensis;circumoval precipitation;circumlarval precipitation;immunodiagnosis

Abstract

Using sera obtained from Angiostrongylus cantonensis-infected or -transferred rats and immunized guinea pigs with A. cantonensis homogenate, positive circumoval and circumlarval precipitate reactions on living A. cantonensis at various developmental stages were recognized after incubation at 34℃ for 24hr. Characteristic precipitates were mainly formed on the excretory pore and cuticle of the 3rd-stage larvae ; the oral opening, excretory pore and cuticle of the 4th-stage larvae ; and the vulva, anus, cloacal and oral openings, excretory pore and cuticle of immature adults. However, precipitates were not formed on the living 1st-stage larvae. Precipitates began to appear 1 to 4 weeks after infection. These reactions were recognized irrespective of the sex and developmental stage of the worm. Positive cross-reactions were observed in the sera from A. costaricensis-infected rats. Applying a technique of immunofluorescence, it was confirmed that immunoglobulins were specifically incorporated into the precipitates. The results of these reactions were compared with those of the indirect hemagglutination and double diffusion tests. For the immunodiagnosis of angiostrongyliasis, the excellency of the circumoval and circumlarval precipitate reactions was noted.

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