Japanese Journal of Veterinary Research;Volume 32, Number 3

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CHARACTERIZATION OF TRANSPLANTABLE SUBLINE MDCC-MSB1-CLO. 18 DERIVED FROM MDCC-MSB1

HIGASHIHARA, Tomoko;KUNIHIRO, Kazuaki;YAMAKI, Tetsuya;OKADA, Ikuo;KODAMA, Hiroshi;IZAWA, Hisao;MIKAMI, Takeshi

Permalink : http://hdl.handle.net/2115/2308
JaLCDOI : 10.14943/jjvr.32.3.155
KEYWORDS : MDCC-MSB1;MDCC-MSB1-Clo. 18;lymphoblastoid cell line;Marek's disease;transplantable cell line

Abstract

MDCC-MSB1-Clo. 18 subline, derived from a Marek's disease (MD) lymphoblastoid cell line MDCC-MSB1,was highly transplantable and lethal for chickens. A dose-response relationship was observed between the number of tumor cells inoculated and the development of tumors in the recipient chickens. In addition to lymphoma formation at the site of transplantation, the recipients developed metastatic lesions in various visceral organs. Age resistance of the chickens to the tumor development was also observed. No tumor development was observed in nude mice inoculated with MDCC-MSB1-Clo. 18 cells. MD virus was isolated from duck embryo fibroblasts inoculated with MDCC-MSB1-Clo. 18 cells and also from kidney cell cultures of MDCC-MSB1-Clo. 18-inoculated chickens. MDCC-MSB1-Clo. 18 cells reacted with anti-B_E and B_K sera in the membrane immunofluorescence test. The tumor cells collected from the chickens also reacted with anti-B_E and B_K sera, while the erythrocytes from these chickens did not react with these antisera. This indicated that the tumors were originated from the inoculated cells.

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