Japanese Journal of Veterinary Research;Volume 33, Number 1-2

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ANALYSIS OF TISSUE CREATINE PHOSPHOKINASE ISOENZYMES BY HISTOELECTROPHORESIS

YASUDA, Jun;SYUTO, Bunei;TOO, Kimehiko

Permalink : http://hdl.handle.net/2115/2316
JaLCDOI : 10.14943/jjvr.33.1-2.19
KEYWORDS : creatine phosphokinase;CPK isoenzyme;tissue;cattle;swine;polyacrylamide disc gel electrophoresis;Veterinary medicine

Abstract

Tissue creatine phosphokinase isoenzymes in cattle and swine were analyzed by histoelectrophoresis. This method is modification of the polyacrylamide disc gel electrophoretic technique of DAVIS and has high practical value compared with previous tissue extracts electrophoretic methods. The skeletal muscle predominantly had MM isoenzyme. The spinal cord had BB and MM isoenzymes. The cardiac muscle had MB and MM isoenzymes. The smooth muscle of cattle had BB and MM isoenzymes. The smooth muscle of swine had BB, MB and MM isoenzymes.

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