Japanese Journal of Veterinary Research;Volume 42, Number 2

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THE INHIBITORY EFFECTS OF MgSO_4 ON THE MULTIPLICATION AND TRANSCRIPTION OF MOUSE HEPATITIS VIRUS

MIZUTANI, Tetsuya;HAYASHI, Masanobu;MAEDA, Akihiko;ISHIDA, Kozue;WATANABE, Tomomasa;NAMIOKA, Shigeo

Permalink : http://hdl.handle.net/2115/2470
JaLCDOI : 10.14943/jjvr.42.2.95
KEYWORDS : MHV;Inhibition;viral multiplication;RNA synthesis;MgSO_4

Abstract

The multiplication of mouse hepatitis virus (MHV) was inhibited by the treatment of infected cells with MgSO_4 at concentrations higher than 50 mM. The inhibition of viral multiplication was more effective with the treatment of cells at the early stage of infection using MgSO_4 than at the late stage. Viral adsorption to the cells was not inhibited by MgSO_4 and pretreatment of the cells with MgSO_4 did not show an inhibitory effect on the RNA synthesis of MHV. The synthesis of viral RNA was inhibited more effectively by the treatment of infected cells with MgSO_4 at 0-2 and 2-4 h postinfection (p.i.) than at 4-6 h p.i. The present study suggests that the stage at which viral multiplication is susceptible to MgSO_4 may be the early stage of viral transcription and that Mg^<2+> may be a useful tool for the analysis of the early stage of MHV infection.

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