Japanese Journal of Veterinary Research;Volume 42, Number 2

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N-TERMINAL AMINO ACID SEQUENCE OF A 28 kDa MAJOR SERUM HIGH DENSITY LIPOPROTEIN OF THE RAINBOW TROUT ONCORHYNCHUS MYKISS

MURATA, Mie;SUGIMOTO, Chihiro;KODAMA, Hiroshi;ONUMA, Misao

Permalink : http://hdl.handle.net/2115/2469
JaLCDOI : 10.14943/jjvr.42.2.89
KEYWORDS : High-density serum lipoprotein;rainbow trout (Oncorhynchus mykiss);salmonid

Abstract

As there are few data on primary structures of fish apolipoproteins (apo), we determined the N-terminal sequence of a 28 kDa major serum protein of rainbow trout, a possible homologue of apoAI protein in higher vertebrates. Rainbow trout serum was separated by two-dimensional electrophoresis. A spot of the 28 kDa protein (pl 5.45) was cut out and directly applied to a protein sequencer. We succeeded in sequencing 30 amino acids from the N-terminal. The sequence of the 28 kDa protein shared a high similarity with that of apoAI of Atlantic salmon (Salmo salar). These results showed that the 28 kDa protein is apoAI of rainbow trout.

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