Japanese Journal of Veterinary Research;Volume 48, Number 4

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A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR

OZAKI, Hiroichi;SUGITA, Shigeo;KIDA, Hiroshi

Permalink : http://hdl.handle.net/2115/2859
JaLCDOI : 10.14943/jjvr.48.4.187

Abstract

A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using imununo-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10^<1.0~2.0> times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10^<7.0~8.0> times higher than those by virus isolation or by RT-PCR.

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