Japanese Journal of Veterinary Research;Volume 50, Number 2-3

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Expression and distribution of inducible nitric oxide synthase in mouse testis

KON, Yasuhiro;NAMIKI, Yuka;ENDOH, Daiji

Permalink : http://hdl.handle.net/2115/2956
JaLCDOI : 10.14943/jjvr.50.2-3.115
KEYWORDS : nitric oxide;testis;immunohistochemistry;Sertoli cells

Abstract

Nitric oxide (NO) is a simple and relatively unstable radical under physiological conditions. It is synthesized by three isoforms of NO synthase, that is neuronal, endothelial and inducible (iNOS) isoforms. In the present study, we investigated the distribution of iNOS with immunohistochemical methods in the mouse testis. The iNOS-immunoreactivity was detected on the basal region of the seminiferous tubules, where the cytoplasm of Sertoli cells was selectively immunolabeled. This immunoreactivity was observed by both immunofluorescent and immunoenzyme methods. Weak immunoreactivity was detected on the perinuclear cytoplasm of Sertoli cells throughout the seminiferous stages, whereas in stages I-VIII, it was remarkable on the processes of Sertoli cells surrounding the spermatogonia and early spermatocytes, and elongating into the lumina of seminiferous tubules. By reverse transcriptase-polymerase chain reaction, mRNA for iNOS was found to be expressed in the mouse testis. These results reveal that iNOS is consistently distributed at the front of the testicular environment.

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