Japanese Journal of Veterinary Research;Volume 50, Number 4

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Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for mtDNA typing in Hokkaido brown bear (Ursus aretos yesoensis)

Satoh, Yuka;Mano, Tsutomu;Tsuruga, Hifumi;Masuda, Ryuichi;Matsuhashi, Tamako;Onuma, Manabu;Suzuki, Masatsugu;Ohtaishi, Noriyuki

Permalink : http://hdl.handle.net/2115/2967
KEYWORDS : Hokkaido brown bear;mitochondrial DNA (mtDNA);PCR RFLP method

Abstract

To develop an easy method of typing of mitochondrial DNA (mtDNA) in Hokkaido brown bears (Ursus arctos yesoensis), the PCR-RFLP technique was improved using four restriction enzymes: Mbo 1, Cfr 13 I, TspE 1, and Fok 1. This approach identified seven groups of mtDNA haplotypes, HB1/2/5-7, HB3, HB4, HB8/9, HB10/11, HB12 and HB13 from 102 brown bears of northern, central and eastern Hokkaido.

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