Japanese Journal of Veterinary Research;Volume 34, Number 3-4

FONT SIZE:  S M L

PURIFICATION AND CHARACTERIZATION OF HEMAGGLUTININ OF CLOSTRIDIUM BOTULINUM TYPE C STRAIN STOCKHOLM

SUZUKI, Norihiko;SYUTO, Bunei;KUBO, Shuichiro

Permalink : http://hdl.handle.net/2115/3023
JaLCDOI : 10.14943/jjvr.34.3-4.269
KEYWORDS : Clostridium botulinum;hemagglutinin purification;hemagglutinin characterization

Abstract

Clostridium botulinum type C hemagglutinin (HA) was purified and characterized for clarifying the function of HA in botulinum intoxication. The purified HA showed a single band on disc electrophoresis and a single precipitin line on agar gel double diffusion test. The specific activity of the purified HA was 1.6×10^4 units/mg protein for rat erythrocytes. The molecular weight of HA was 230,000 by ultracentrifuge analysis. HA bound not only to erythrocytes but also to intestinal cells. The HA activity was inhibited by the addition of gangliosides (GM_3,GM_4,GD_<1a>, GD_<1b>, and GT_<1b>) and fetuin, and was not shown to erythrocytes treated previously by neuraminidase. These results suggest that HA binds to sialic acid on cell surface. In botulinum intoxication, HA may play as a carrier for the internalization of toxin through small intestine cell.

FULL TEXT:PDF