Japanese Journal of Veterinary Research;Volume 38, Number 1

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LACTATE DEHYDROGENASE AND CREATINE PHOSPHOKINASE ISOENZYMES IN TISSUES OF LABORATORY ANIMALS

YASUDA, Jun;TATEYAMA, Kazue;SYUTO, Bunei;TOO, Kimehiko

Permalink : http://hdl.handle.net/2115/3192
KEYWORDS : lactate dehydrogenase (LDH);creatine phosphokinase (CPK);isoenzyme;tissue;laboratory animal

Abstract

The lactate dehydrogenase (LDH) and creatine phosphokinase (CPK) isoenzyme distributions in tissues of the ICR mouse, Wistar rat, guinea pig and golden hamster were analyzed by histoelectrophoresis. Tissues obtained were as follows : liver, pancreas, stomach, small intestine, heart, femoral muscle, uterus, kidney, spleen, lymph node, cerebrum, spinal cord and erythrocyte. Histoelectrophoresis was for the direct analysis of LDH and CPK isoenzymes in the tissues and had high practical value compared with previous tissue-extraction methods. In tissues of the mouse, guinea pig and golden hamster, LDH isoenzymes showed five bands. In the rat, LDH isoenzyme was separated into four fractions. CPK isoenzyme showed three bands ; BB, MB and MM. In some tissues, the MM band was separated into two sub fractions.

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