Japanese Journal of Veterinary Research;Volume 38, Number 1

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CHARACTERIZATION OF TWO MONOCLONAL ANTIBODIES WHICH RECOGNIZE DIFFERENT SUBPOPULATIONS OF CHICKEN T LYMPHOCYTES

KONDO, Takashi;HATTORI, Masakazu;KODAMA, Hiroshi;ONUMA, Misao;MIKAMI, Takeshi

Permalink : http://hdl.handle.net/2115/3191
JaLCDOI : 10.14943/jjvr.38.1.11
KEYWORDS : Chicken;monoclonal antibody;subpopulation;T lymphocyte

Abstract

Distribution among peripheral T lymphocyte subpopulations and biochemical properties of the chicken lymphocyte surface antigens defined by monoclonal antibodies (mAbs) Lc-4 and Lc-6 were examined. Two-color immunofluorescence analysis revealed that Lc-4 and Lc-6 antigens were expressed on mutually exclusive subpopulations of peripheral T lymphocytes but not on B lymphocytes. Lc-4 mAb precipitated a polypeptide with apparent molecular mass of 35 and 65 kilodalton under reducing and non-reducing conditions, respectively. These results indicated that the antigen recognized with Lc-4 was closely similar in tissue distribution and biochemical property to mammalian CD8 antigen.

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