Japanese Journal of Veterinary Research;Volume 8, Number 1-4

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A STUDY ON THE MEASUREMENT OF THE VOLUME OF THE CIRCULATORY BLOOD OF THE HORSE

MISE, Hideo

Permalink : http://hdl.handle.net/2115/4673
JaLCDOI : 10.14943/jjvr.8.1-4.74

Abstract

Studies on methods of measurement of circulatory blood volume and circulatory plasma volume of horses by the dye method and the P method were made on 32 healthy horses; the following summarized results were obtained. 1) When values obtained by LAWSON, GREGERSEN, and GIBSON & EVANS using T-1824 were compared, within a range of 0.202∿0.289mg per kg body weight GIBSON & EVANS' method showed the highest values in both circulatory blood volume and circulatory plasma volume. Circulatory blood volume per kg body weight obtained by LAWSON'S method was 62.0ml, by GREGERSEN'S 72.2ml, by GIBSON & EVANS' 76.0ml. Circulatory plasma volume by LAWSON'S was 40.0ml, by GREGERSEN'S 46.4ml, by GIBSON & EVANS' method 49.0ml. 2) When circulatory blood volume and circulatory plasma volume changes corresponding to dye volume changes within a range of 0.202∿0.586mg were studied, within the range 0.202∿0.250 no significant changes were seen. However when the volume of infused dye was increased, the values of circulatory blood volume and plasma volume increased with the increase of volume of infused dye. 3) Dye was infused through jugular vein and blood samples were taken at regular intervals from the opposite jugular vein and right forefoot median vein; the differences by sampling site were studied. It was found in regard to the time course of extinction and the circulatory blood volume and plasma volume obtained from the time course of extinction, that no significant differences were seen by sampling site. 4) From the correlation between circulatory blood volume and circulatory plasma volume and body weight obtained from infused dye volume within a range of 0.202∿0.289mg per kg body weight by least square method, circulatory blood volume and circulatory plasma volume per kg body weight calculated. 5) One minute values of extinction were calculated by LAWSON's method, by extrapolation to the vertical axis. When extrapolated to the horizontal axis the time of disappearance was obtained. Since an experimental formula can be expressed by least square method when the time of disappearance is calculated by this formula, the time required at its shortest was 7 days and the same at its longest was 260 days. However, in actuality when long term observations were made on plasma dye concentration it was found that the time of disappearance was 5∿6 days. 6) When circulatory blood volume was measured, samples were taken at regular intervals; Ht values were measured from part of the samples and their changes were studied. It was noted that besides experimental error, considerable physiological changes occurred. 7) In the measurement of circulatory blood volume by P especially in blood corpuscle method the total blood volume value was 73.56ml per kg body weight while by use of total blood method the obtained value was 46.95ml; thus the blood corpuscle method showed higher values than total blood method. When the P method and the dye method were compared, it was noted that the values by GREGERSEN'S method were closely approximate to those obtained by the P method. When compared with simultaneous measurement by T-1824,it was shown that in both circulatory blood volume and circulatory plasma volume, GREGERSEN'S and GIBSON & EVANS' methods showed higher values than those obtained by P method while by LAWSON's method the values were smaller. The author wishes to acknowledge the valuable guidance and assistance of Prof. Keizo HONMA.

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