Japanese Journal of Veterinary Research;Volume 8, Number 1-4




Permalink : http://hdl.handle.net/2115/4674
JaLCDOI : 10.14943/jjvr.8.1-4.107


The result of this research may be summarized as follows : 1) Connective tissue fibers appearing in the above described 6 groups of structures of pig ovaries were observed with the use of GOMORI's silver impregnation method. 2) Normal vesicular follicles were divided into 2 types. 3) Normal vesicular follicles in the process of maturation could not be distinguished by the findings of silver preparations : fibrous elements are not found in the granulosa layer. The theca interna consists of only argyrophil fibers, which form a meshwork. The theca externa is composed of collagenous fibers, which run to parallel to the follicle wall and do not tend to form a meshwork. 4) In mature follicles and in just before ovulation the behavior of the connective tissue fibers in the walls is similar to that of follicles in maturating process. But in these follicles small masses of similar networks to these in the theca interna protrude from the inner side of the theca interna into the granulosa layer, showing plica-like configuration. 5) Cystic atretic follicles were divided into 9 types. 6) At the earliest stage of the regressive process the behavior of connective tissue fibers is almost like that in normal vesicular follicles. At the more advanced and the middle stages in a meshwork of argyrophil fibers of theca interna, collagenous fibers appear and increase gradually. Then after the appearance and gradual development of the yellow body-like tissues in the periphery of the follicle cavity, fine argyrophil fibers penetrate into these tissues from the theca interna, and gradually form a fine meshwork. Accompanied by the appearance and increasing of hyaline layers in the inner side of the theca interna, fine granular substances come into sight and increase in quantity. Moreover, with the appearance of radial layers in the outer wall of atretic follicles, collagenous fibers of these parts increase and form large networks in radial arrangement. At the last stage, in the scar tissue, the small zigzag folds composed of fine granular substances are found in parts corresponding to so-called glass membrane. In the outer side of this scar, radially arranged networks begin to decrease and argyrophil fibers which filled out the original follicle cavity show gradual transformation into collagenous fibers. 7) In this research there were found some follicles which were recognized to be undergoing change into the regressive process after maturation. 8) Atretic follicles with hemorrhage were divided into 4 types in this research. 9) In this research these follicles at the early stage of regression were found to be similar to the normal vesicular follicles, and those at the last stage were also like in character to the last stage of cystic atretic follicles. At the middle stage, however, slight differences were found in the connective tissue fibers of the wall, in spite of the active penetrating and increasing of the argyrophil fibers into the follicle cavity. 10) Corpora lutea of non-pregnancy were divided into 9 types. 11) At the vascularization stage fine argyrophil fibers penetrate actively from theca lutein cell layers into granulosa lutein cell layers, and form networks enclosing 2 or more granulosa lutein cells in one of the same networks. At the bloom stage these fibers encircle completely each lutein cell. At the earliest stage of regression the condition of argyrophil fibers is similar to that of the bloom stage. But at a later stage collagenous fibers appear and increase gradually around lutein cells contrary to the diminishing of argyrophil fibers. And fine pale purple fibers or fine pale purple granular structures come into sight and develop gradually in the parts which are undergoing hyaline degeneration. At the last stage the whole wall of a corpus luteum becomes occupied by fine pale purple granular substances, in which a few broken argyrophil and swollen collagenous fibers are scattered. 12) On the derivation of lutein cells, the present writer in this research has confirmed definitely the dualistic theory of CORNER. 13) Corpora lutea of non-pregnancy with a large central cavity were divided into 6 types. 14) In this research the behavior of the connective tissue fibers in the wall was found to be similar to that of corpora lutea of non-pregnancy. 15) "Vascular bodies" were divided into 3 types. 16) In the regressive process the connective tissue fibers show similar processes of change as in corpora lutea of non-pregnancy. In this research, however, argyrophil fibers in the wall of blood vessels, especially arterioles are maintained in the far advanced stage, showing the characteristics of the present writer's so-called "vascular bodies." The writer wishes to express his gratitude to Prof. K. TAKAHATA, the chief of the Department of Veterinary Anatomy, for his kind instructions and reviews. Further he would like to express his cordial thanks to the members of the staff of the Department who assisted on this research.