Japanese Journal of Veterinary Research;Volume 8, Number 1-4

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GROWTH OF CLOSTRIDIUM BOTULINUM TYPE E (IWANAI) IN SEMI-SYNTHETIC MEDIUM

INUKAI, Yoshikazu;HAGA, Tadashi

Permalink : http://hdl.handle.net/2115/4675
JaLCDOI : 10.14943/jjvr.8.1-4.127

Abstract

As the first step to study the mechanism of toxin production by Cl. botulinum type E (Iwanai), semi-snythetic medium containing casamino acids as nitrogen source was prepared. For sufficient growth, at least casamino acids, tryptophan, cysteine, thioglycollate, glucose, inorganic salts, nicotinamide, biotin, pyridoxine, thiamine, folic acid and Ca-pantothenate were necessary. In this medium, the growth of micro-organisms reached to about 70 per cent as in liver-broth, while the production of toxin was only below 1/10 as compared with yields in liver-broth.

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