Japanese Journal of Veterinary Research;Volume 32, Number 2

FONT SIZE:  S M L

IMMUNOHISTOCHEMICAL STUDIES ON THE LOCALIZATION OF ANTIGEN-BEARING PHAGOCYTES AND LYMPHOCYTES IN MOUSE LYMPH NODES AFTER THE GERMINAL CENTER FORMATION

NAKAMURA, Kazuichi;HASHIMOTO, Yoshiharu;KITAGAWA, Hiroshi;KUDO, Norio

Permalink : http://hdl.handle.net/2115/4694
JaLCDOI : 10.14943/jjvr.32.2.73
KEYWORDS : antigen-bearing phagocytes;lymphocytes;germinal centers;mouse lymph nodes

Abstract

The antigen, 2,4-dinitrophenylated and alumprecipitated bovine serum albumin, was inoculated into the foot pads at 2 weeks after the primary inoculation of the antigen. At 2 and 3 days after the second inoculation, many anti-T cell serum negative lymphocytes, possibly B cells, were found in the lumen and the endothelium of the post capillary venules, and they predominantly gathered around the venules. These gathering areas of possibly B cells were encountered frequently in contact with the cortical lymphatic nodules containing the germinal centers. From 4 to 14 days after the second inoculation, a number of antigen-bearing phagocytes appeared in the cortical marginal zones, which were located between the germinal centers and the marginal sinuses. These findings suggest that B cells are largely recruited from the blood to the paracortex, and also the antigen-bearing phagocytes directly present the antigen to the cortical lymphocytes for the acceleration of the antibody production after the formation of the germinal centers.

FULL TEXT:PDF