Permalink : http://hdl.handle.net/2115/54819

KEYWORDS : gas chromatography;fatty acid;ionic liquid;SLB-IL111;equivalent chain length;fish oil

This paper reports the retention data of fatty acid methyl esters on a novel ionic liquid column, SLB-IL111, which has higher polarity than commercially available columns for gas chromatography (GC). The fatty acids analyzed using GC include branched-chain saturated fatty acids, C16-C22 monounsaturated fatty acid cis/trans and positional isomers, and polyunsaturated fatty acids commonly found in natural products. Fatty acids of sardine oil were also analyzed using GC. GC was carried out on a 100-m column under isothermal conditions within the range 120-170°C, using helium as a carrier gas. The retention data of fatty acid methyl esters were represented by equivalent chain length (ECL) values calculated from the retention times of each fatty acid related to those of saturated fatty acids with an even carbon number. This is novel information, obtained on an SLBIL111 column being operated at various temperatures with a single GC system. The results show that the column temperature greatly affected the ECL values of unsaturated fatty acids. Thus, when using this column for the analysis of fish oil fattya cids, it is necessary to precisely control the column temperature.