Japanese Journal of Veterinary Research;Volume 64 Supplement 2


Molecular profile of some Bacillus cereus strains isolated from milk with reference to its susceptibility to lysozyme

Suelam, Iman I. A.;Merwad, Abdallah M. A.;Mohamed, Mohamed E. M.

Permalink : http://hdl.handle.net/2115/62019
KEYWORDS : Bacillus cereus;bacteria;ERIC-PCR;lysozyme


The present study aimed to clarify the molecular diversity of B. cereus isolated from milk and its susceptibility to lysozyme. A total of 153 individual raw milk samples were randomly collected. Bacteriological examination was carried out according to standard methods. In vitro susceptibility of Bacillus species to lysozyme was done for all isolates (No=153). ERICPCR fingerprinting was done for 10 B. cereus strains. It was found that 61 (39.9%) of milk samples were positive for B. cereus. Susceptibility to lysozyme was the highest in B. cereus (47.5%) among other Bacillus species. Molecular fingerprinting produced patterns of 11 major bands. Dendrogram revealed 3 main clusters. The highest degree of similarity was noticed among isolates in different date of isolation. It could be concluded from this study that the isolated B. cereus clones had diversity in terms of molecular profile and sensitivity to lysozyme.