Japanese Journal of Veterinary Research;Volume 64 Supplement 2


Occurrence of Bacillus cereus in raw milk and some dairy products in Egypt

Mohamed, Asmaa S.;Alnakip, Mohamed E. A.;Aal, Salah F. Abd-El

Permalink : http://hdl.handle.net/2115/62020
KEYWORDS : Bacillus cereus;raw milk;dairy products;Food-poisoning;Egypt


A total of 160 samples (20 each of raw cow's milk and seven dairy products; pasteurized milk, small and large scales-manufactured ice cream, infant formulas, full-fat milk powder, rice pudding and yoghurt) were investigated for the presence of B. cereus. Biochemical tests followed by PCR were carried out to confirm the suspected B. cereus isolates. Overall 44 samples yielded Bacillus-like growth of which 34 were positive for B. cereus. The highest occurrence % of B. cereus was detected in raw cow's milk (60 %), and rice pudding (55 %). Non-B. cereus isolates were identified based on 16S rRNA GS and 7 isolates of B. licheniformis and 3 isolates of B. subtilis were obtained. No psychrotolerant B. cereus strains were detected in all samples. Evidence evolved from our results confirms the need for authorities and producers to adopt B. cereus as a reference in microbiological hazard control, particularly for dairy products.