Japanese Journal of Veterinary Research;Volume 64 Supplement 2


Optimization of the isolation process of putative bovine mammary stem cells

Abdelfattah-Hassan, Ahmed;Saadeldin, Islam M.;Ghonimi, Wael

Permalink : http://hdl.handle.net/2115/62024
KEYWORDS : MaSC;Isolation protocol;Immunofluorescence;Collagen;dissociation time


Mammary gland stem cells are a dormant cell population inside the mammary gland that are cable of differentiation into its all cell types. Increased knowledge on bovine mammary stem cells is of useful importance to the dairy industry, as they are important to mammary gland development and tissue maintenance. Also, bovine mammary stem cells seem a good model for human breast cancer, since mammary cancer is not observed in cows. Proper isolation technique for the putative mammary stem cells is an essential step for the succeeding approaches and experiments using these cells. Therefore, this study was designed to evaluate different steps for the optimum isolation of putative bovine mammary stem cells. This included sites for sampling from the Bovine mammary gland, tissue dissociation steps and different culturing conditions. The optimum conditions for isolating and culturing putative bovine mammary stem cells were sampling as near to the base of the quarter as possible and using 1 hour dissociation step, followed by culturing the cells in tissue culture flasks coated with 100 μg/mL bovine collagen type-1.